Device

Part:BBa_K4646018:Design

Designed by: Alexander Diefes   Group: iGEM23_Duke   (2023-10-10)


EF1alpha _Kozak_IL-2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 643
    Illegal XhoI site found at 1042
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 777
    Illegal AgeI site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part needs to be linked with the N-terminus of the secreted protein.


Source

This construct contains mammalian sequences.

References